Article No
LS-C358016-100
TP53 / p53
P53 antibody LS-C358016 is an unconjugated mouse monoclonal antibody to p53 (TP53) from human. It is reactive with human, bovine and monkey. Validated for IHC and WB.
Antibodies Primary
Specifications
| NCBI Number | 7157 |
| Application | IHC, WB |
| Article No | LS-C358016-100 |
| Country Availability | SE, FI, DK, NO, FO, GL |
| Clone | DO-7 |
| Clone Type | monoclonal |
| Concentration | 0.2 mg/ml |
| Conjugation | Unconjugated |
| Description | P53 antibody LS-C358016 is an unconjugated mouse monoclonal antibody to p53 (TP53) from human. It is reactive with human, bovine and monkey. Validated for IHC and WB. |
| Recommended Dilution | IHC-P (0.5 - 1 ug/ml), WB (0.5 - 1 ug/ml) |
| Supplier | LifeSpan Biosciences |
| Entrez Gene ID | 7157 |
| Gene Symbol | TP53 |
| Immunogen | Recombinant human wild type p53 expressed in E. coli. |
| Isotype | IgG2b k |
| Notes | P53 antibody LS-C358016 is an unconjugated mouse monoclonal antibody to p53 (TP53) from human. It is reactive with human, bovine and monkey. Validated for ELISA, Flow, IF, IHC, IP and WB. |
| Alias Names | TP53, BCC7, Cellular tumor antigen p53, p53, TRP53, p53 tumor suppressor, Phosphoprotein p53, Antigen NY-CO-13, Tumor protein p53, Tumor suppressor p53, LFS1 |
| Product Type | Antibodies Primary |
| Protocol | This antibody is specific for a 53kDa protein, which is identified as p53 suppressor gene product. The large number of prolines in its amino acid sequence causes p53 to migrate slowly in SDS-PAGE, resulting in the amino acid content-estimated 43 kDa protein appearing larger than expected. DO-7 reacts with the mutant as well as the wild form of p53 under denaturing and non-denaturing conditions. It binds to MDM2, SV40 T antigen and human papilloma virus E6 protein. Positive nuclear staining with p53 antibody has been reported to be a negative prognostic factor in breast carcinoma, lung carcinoma, colorectal, and urothelial carcinoma. p53 positivity has also been used to differentiate uterine serous carcinoma from endometrioid carcinoma as well as to detect intra-tubular germ cell neoplasia. ELISA: Order BSA-free antibody format for coating. |
| Purification | Protein G Purified |
| Shipping Information | RT |
| Size | 100 µg |
| Source / Host | mouse |
| Species Reactivity | cow/bovine, human, monkey |
| Storage | 4°C |
| Substrate / Buffer | PBS, pH 7.4, 0.1 mg/ml BSA, 0.05% Sodium Azide |
| Technical Specifications | Human TP53 / p53 |
| Product Page Updated | 2024-04-12T09:20:06.597Z |
