Article No
LS-C389420-100
Actin
Actin antibody LS-C389420 is an unconjugated mouse monoclonal antibody to Actin (N-Terminus) from human. It is reactive with human, mouse, rat and other species. Validated for ELISA, Flow, IF, IHC, IP and WB.
Antibodies Primary
Specifications
| Application | ELISA, FC, IF, IHC, IP, WB |
| Article No | LS-C389420-100 |
| Country Availability | SE, FI, DK, NO, FO, GL |
| Clone | 1A4 |
| Clone Type | monoclonal |
| Concentration | 1 mg/ml |
| Conjugation | Unconjugated |
| Description | Actin antibody LS-C389420 is an unconjugated mouse monoclonal antibody to Actin (N-Terminus) from human. It is reactive with human, mouse, rat and other species. Validated for ELISA, Flow, IF, IHC, IP and WB. |
| Recommended Dilution | FC (0.5 - 1 ug/10e6 cells), IF (0.5 - 1 ug/ml), IHC-P (0.5 - 1 ug/ml) |
| Supplier | LifeSpan Biosciences |
| Immunogen | An N-Terminus decapeptide of the alpha smooth muscle isoform. |
| Isotype | IgG2a k |
| Notes | Actin antibody LS-C389420 is an unconjugated mouse monoclonal antibody to Actin (N-Terminus) from human. It is reactive with human, mouse, rat and other species. Validated for ELISA, Flow, IF, IHC, IP and WB. |
| Product Type | Antibodies Primary |
| Protocol | The applications listed have been tested for the unmodified form of this product. Other forms have not been tested. Actin is a major component of the cytoskeleton and is present in most cell types. This Smooth muscle actin antibody is highly specific to the smooth muscle form. Its epitope lies in the first four N-terminal amino acids. The Smooth muscle actin antibody does not stain cardiac or skeletal muscle. however, it does stain myofibroblasts and myoepithelial cells. It could be used together with antimuscle specific actin and myogenin in making a diagnosis of smooth/skeletal muscle tumors. In most cases of rhabdomyosarcoma, this smooth form antibody yields negative results whereas antimuscle specific actin and myogenin are positive. Leiomyosarcomas are positive only with antimuscle specific actin and are negative with anti-myogenin. No special pretreatment is required for the immunohistochemical staining of FFPE tissue. ELISA: Order BSA-free format. |
| Purification | Protein G Purified |
| Shipping Information | RT |
| Size | 100 µg |
| Source / Host | mouse |
| Species Reactivity | cat/feline, chicken, cow/bovine, dog/canine, goat, guinea pig, human, monkey, mouse, pig/porcine, rabbit, rat, sheep/ovine |
| Storage | 4°C |
| Substrate / Buffer | Sterile Solution PBS |
| Technical Specifications | Human Actin |
| Product Page Updated | 2024-04-12T09:20:06.597Z |
