Article No
LS-C389893-100
CD45RA
CD45RA antibody LS-C389893 is an unconjugated mouse monoclonal antibody to human CD45RA. Validated for Flow, IF and IHC.
Antibodies Primary
Specifications
| Application | FC, IF, IHC |
| Article No | LS-C389893-100 |
| Country Availability | SE, FI, DK, NO, FO, GL |
| Clone | 111-1C5 |
| Clone Type | monoclonal |
| Concentration | 0.2 mg/ml |
| Conjugation | Unconjugated |
| Description | CD45RA antibody LS-C389893 is an unconjugated mouse monoclonal antibody to human CD45RA. Validated for Flow, IF and IHC. |
| Recommended Dilution | FC (0.5 - 1 ug/10e6 cells), IF (1 - 2 ug/ml), IHC-P (0.5 - 1 ug/ml) |
| Supplier | LifeSpan Biosciences |
| Immunogen | Stimulated human leukocytes were used as immunogen for this CD45RA antibody. |
| Isotype | IgG1 k |
| Notes | CD45RA antibody LS-C389893 is an unconjugated mouse monoclonal antibody to human CD45RA. Validated for Flow, IF and IHC. |
| Product Type | Antibodies Primary |
| Protocol | CD45RA is an isoform of the human leukocyte common antigen (CD45). Human CD45 contains three exons which encode peptide segments designated A, B and C, respectively. The differential splicing of the exons generates at least five isoforms, ABC, AB, BC, B and O. This antibody reacts with ABC and BC isoforms. CD45RA is expressed on 40-50% of peripheral CD4+ T-cells, 50% of peripheral CD8+ T-cells, B-cells, and leukemic B-cell lines. T-cells expressing CD45RA are naive or virgin T-cells. T-cells expressing CD45RO are memory T-cells. CD45RA and CD45RO define complementary, predominantly non-overlapping populations of resting peripheral T-cells. This MAb is useful in study on the subpopulation of CD4+ or CD8+ T-cells. It can especially be used to differentiate T-cell lymphomas (CD45RO +ve) from B cell lymphomas (CD45RA +ve). Due to variation in protocol and secondary antibody used, the CD45RA antibody may need to be titered for optimal performance. FFPE staining requires boiling tissue sections in 10mM citrate buffer, pH 6.0, for 10-20 min followed by cooling at RT for 20 minutes. |
| Purification | Protein G Purified |
| Shipping Information | RT |
| Size | 100 µg |
| Source / Host | mouse |
| Species Reactivity | human |
| Storage | 4°C |
| Substrate / Buffer | 0.01% BSA, 0.05% sodium azide |
| Technical Specifications | Human CD45RA |
| Product Page Updated | 2024-04-12T09:20:06.597Z |
