Antibodies Secondary

Mouse Anti-Human IgA Secondary Antibody (clone SPM187)

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Mouse Anti-Human IgA Secondary Antibody (clone SPM187)

Article No

LS-C390402-20

Application

FC, IF, IHC

Species Reactivity

human

Size

20 µg

Clone

SPM187

Source / Host

mouse

Shipping Information

RT

Suggested protocols

Specifications

Application FC, IF, IHC
Article No LS-C390402-20
Country Availability SE, FI, DK, NO, FO, GL
Clone SPM187
Clone Type monoclonal
Concentration 0.2 mg/ml
Conjugation Unconjugated
Description Mouse Anti-Human IgA Secondary Antibody (clone SPM187)
Recommended Dilution FC (1 - 2 µg/10e6 cells), IF (0.5 - 1 µg/ml), IHC-P (0.5 - 1 µg/ml)
Supplier LifeSpan Biosciences
Immunogen Purified human IgA
Isotype IgG1 k
Notes Human IgA antibody LS-C390402 is an unconjugated mouse monoclonal antibody. Validated for Flow, IF and IHC.
Product Type Antibodies Secondary
Protocol This mAb is specific to heavy chain of IgA and shows minimal cross-reaction with heavy chains of other immunoglobulins. It is reactive with both IgA1 and IgA2 subclasses of Alpha heavy chain. It reacts with the third constant domain (CH3) of the alpha chain of IgA molecules. Immunoglobulins are four-chain, Y-shaped, monomeric structures comprised of two identical heavy chains and two identical light chains held together through inter-chain disulfide bonds. The chains form two domains, the Fab (antigen binding) fragment and the Fc (constant) fragment. Immunoglobulin A (IgA) is the main protein of the mucosal immune system. It is generated by B-cells in gut-associated lymphoid tissues. Daily production of IgA exceeds that of any of the other immunoglobulins. IgA exists mainly in dimers but can also exist as polymers or as monomers. Dimers and polymers contain a joining (J) chain that can be bound by the polymeric immunoglobulin receptor (pIgR) for transportation of the molecule to mucosal surfaces. The most common feature of plasmacytomas, and certain non-Hodgkin s lymphomas is the restricted expression of a single heavy chain class. Demonstration of clonality in lymphoid infiltrates indicates that the infiltrate is clonal and therefore malignant. Optimal dilution of the Anti-IgA antibody should be determined by the researcher. Staining of formalin-fixed tissues requires boiling tissue sections in 10mM Citrate buffer, pH 6.0, for 10-20 min followed by cooling at RT for 20 min
Purification Protein G Purified
Shipping Information RT
Size 20 µg
Source / Host mouse
Species Reactivity human
Storage Store at 2°C to 8°C.
Substrate / Buffer 0.01% BSA, 0.05% sodium azide
Technical Specifications Human IgA
Product Page Updated 2021-04-27T14:00:34.181Z

Shipping info

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