Antibodies Secondary

Mouse Anti-Human IgM Secondary Antibody (clone IM260 + IM373 + ICO-30, Azide-free)

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Mouse Anti-Human IgM Secondary Antibody (clone IM260 + IM373 + ICO-30, Azide-free)

Article No

LS-C390418-100

Application

FC, IF, IHC, WB

Species Reactivity

human

Size

100 µg

Clone

IM260 + IM373 + ICO-30

Source / Host

mouse

Shipping Information

RT

Specifications

Application FC, IF, IHC, WB
Article No LS-C390418-100
Country Availability SE, FI, DK, NO, FO, GL
Clone IM260 + IM373 + ICO-30
Clone Type monoclonal
Concentration 1 mg/ml
Conjugation Unconjugated
Description Mouse Anti-Human IgM Secondary Antibody (clone IM260 + IM373 + ICO-30, Azide-free)
Recommended Dilution FC (0.5 - 1 µg/10e6 cells), IF (0.5 - 1 µg/ml), IHC-P (0.5 - 1 µg/ml), WB (0.5 - 1 µg/ml)
Supplier LifeSpan Biosciences
Immunogen Recombinant heavy chain of human IgM (IM260 IM373 & ICO-30).
Isotype IgG1, IgG2b k
Notes Human IgM antibody LS-C390418 is an unconjugated mouse monoclonal antibody. Validated for Flow, IF, IHC and WB.
Product Type Antibodies Secondary
Protocol The applications listed have been tested for the unmodified form of this product. Other forms have not been tested. Recognizes a protein of 75kDa, identified as mu heavy chain of human immunoglobulins. It does not cross-react with alpha (IgA), gamma (IgG), epsilon (IgE), or delta (IgD), heavy chains, T-cells, monocytes, granulocytes, or erythrocytes. This mAb is useful in the identification of leukemias, plasmacytomas, and certain non-Hodgkins lymphomas. The most common feature of these malignancies is the restricted expression of a single heavy chain class. Demonstration of clonality in lymphoid infiltrates indicates that the infiltrate is clonal and therefore malignant. Optimal dilution of the Anti-IgM antibody cocktail should be determined by the researcher. Staining of formalin-fixed tissues requires boiling tissue sections in 10mM Citrate buffer, pH 6.0, for 10-20 min followed by cooling at RT for 20 min.
Purification Protein G Purified
Shipping Information RT
Size 100 µg
Source / Host mouse
Species Reactivity human
Storage Aliquot and store at -20°C. Avoid freeze-thaw cycles.
Substrate / Buffer Sterile Solution PBS
Technical Specifications Human IgM
Product Page Updated 2021-04-27T14:00:34.181Z

Shipping info

The delivery time for this item is approximately 9-18 business days. If other deviations occur, this will be noted on the order confirmation or communicated via email. Please note, we do reserve the right to select the best packaging and shipping method in order to insure the stability of the product and allow efficient order tracking.