Article No
LS-C391577-20
EMA / MUC1
MUC1 antibody LS-C391577 is an unconjugated mouse monoclonal antibody to human MUC1 (EMA). Validated for Flow, IF and IHC.
Antibodies Primary
Specifications
| NCBI Number | 4582 |
| Application | FC, IF, IHC |
| Article No | LS-C391577-20 |
| Country Availability | SE, FI, DK, NO, FO, GL |
| Clone | E29 |
| Clone Type | monoclonal |
| Concentration | 0.2 mg/ml |
| Conjugation | Unconjugated |
| Description | MUC1 antibody LS-C391577 is an unconjugated mouse monoclonal antibody to human MUC1 (EMA). Validated for Flow, IF and IHC. |
| Recommended Dilution | FC (0.5 - 1 ug/10e6 cells), IF (0.5 - 1 ug/ml), IHC-P (0.1 - 0.2 ug/ml) |
| Supplier | LifeSpan Biosciences |
| Entrez Gene ID | 4582 |
| Gene Symbol | MUC1 |
| Immunogen | Delipidated extract of human milk fat globule membranes. |
| Isotype | IgG2a k |
| Notes | MUC1 antibody LS-C391577 is an unconjugated mouse monoclonal antibody to human MUC1 (EMA). Validated for Flow, IF and IHC. |
| Alias Names | MUC1, CA15-3, CD227, Carcinoma-associated mucin, CD227 antigen, DF3 antigen, Episialin, Epithelial membrane antigen, H23 antigen, H23AG, Krebs von den Lungen-6, MAM6, MUC1/ZD, MUC-1/SEC, Mucin-1, Pem, KL-6, MUC-1/X, Tumor-associated mucin, Peanut-reactive urinary mucin, Polymorphic epithelial mucin, PUM, EMA, MUC-1, Mucin 1, transmembrane |
| Product Type | Antibodies Primary |
| Protocol | In Western blotting, it recognizes proteins in MW range of 265-400kDa, identified as different glycoforms of EMA. EMA may provide a protective layer on epithelial cells against bacterial and enzyme attack. In immunohistochemical assays, it superbly stains routine formalin/paraffin carcinomas. Antibody to EMA is useful as a pan-epithelial marker for detecting early metastatic loci of carcinoma in bone marrow or liver. The optimal dilution of the MUC1 antibody for each application should be determined by the researcher. Staining of formalin-fixed tissues requires boiling tissue sections in 10mM citrate buffer, pH 6.0, for 10-20 min followed by cooling at RT for 20 minutes. |
| Purification | Protein G Purified |
| Shipping Information | RT |
| Size | 20 µg |
| Source / Host | mouse |
| Species Reactivity | human |
| Storage | 4°C |
| Substrate / Buffer | PBS, 0.01% BSA, 0.05% sodium azide |
| Technical Specifications | Human EMA / MUC1 |
| Product Page Updated | 2024-04-12T09:20:06.597Z |
