Article No
LS-C392260-20
Mouse Anti-Human Kappa Light Chain Secondary Antibody (clone SPM558)
Human Kappa Light Chain antibody LS-C392260 is an unconjugated mouse monoclonal antibody. Validated for Flow, IF, IHC and WB.
Antibodies Secondary
Specifications
| Application | FC, IF, IHC, WB |
| Article No | LS-C392260-20 |
| Country Availability | SE, FI, DK, NO, FO, GL |
| Clone | SPM558 |
| Clone Type | monoclonal |
| Concentration | 0.2 mg/ml |
| Conjugation | Unconjugated |
| Description | Human Kappa Light Chain antibody LS-C392260 is an unconjugated mouse monoclonal antibody. Validated for Flow, IF, IHC and WB. |
| Recommended Dilution | FC (0.5 - 1 ug/10e6 cells), IF (0.5 - 1 ug/ml), IHC-P (0.5 - 1 ug/ml), WB (0.5 - 1 ug/ml) |
| Supplier | LifeSpan Biosciences |
| Immunogen | Recombinant human Ig kappa chain. |
| Isotype | IgG1 k |
| Notes | Human Kappa Light Chain antibody LS-C392260 is an unconjugated mouse monoclonal antibody. Validated for Flow, IF, IHC and WB. |
| Alias Names | , IgK, Ig Kappa |
| Product Type | Antibodies Secondary |
| Protocol | This mAb is specific to kappa light chain of immunoglobulin and shows no cross-reaction with lambda light chain or any of the five heavy chains. In mammals, the two light chains in an antibody are always identical, with only one type of light chain, kappa or lambda. The ratio of Kappa to Lambda is 70:30. However, with the occurrence of multiple myeloma or other B-cell malignancies this ratio is disturbed. Antibody to the kappa light chain is reportedly useful in the identification of leukemias, plasmacytomas, and certain non-Hodgkin's lymphomas. Demonstration of clonality in lymphoid infiltrates indicates that the infiltrate is malignant. The optimal dilution of the anti-Kappa antibody for each application should be determined by the researcher. Staining of formalin-fixed tissues requires boiling tissue sections in 10mM Citrate Buffer, pH 6.0, for 10-20 min followed by cooling at RT for 20 minutes. |
| Purification | Protein G Purified |
| Shipping Information | RT |
| Size | 20 µg |
| Source / Host | mouse |
| Species Reactivity | human |
| Storage | 4°C |
| Substrate / Buffer | 0.01% BSA, 0.05% sodium azide |
| Technical Specifications | Human Kappa Light Chain |
| Product Page Updated | 2024-04-12T09:20:06.597Z |
