Article No
M087-3
Anti-Caspase-3 (Human) mAb
Anti-Caspase-3 (Human) mAb
Antibodies Primary
Specifications
| Application | IHC, WB |
| Article No | M087-3 |
| Country Availability | SE, FI, DK, NO, IS, EE, LV, LT, FO, GL |
| Clone | 1F9 |
| Clone Type | monoclonal |
| Concentration | 1 mg/mL |
| Conjugation | Unconjugated |
| Description | Anti-Caspase-3 (Human) mAb |
| Supplier | MBL |
| Immunogen | Recombinant human caspase-3 |
| Isotype | IgG1 |
| Notes | Caspase-3 (also known as CPP32, Yama, apopain) is a key member of the caspase family of cysteine proteases. Caspase-3 exists in cells as an inactive 32 kDa proenzyme. During apoptosis procaspase-3 is processed at aspartate residues by self-proteolysis and/or cleavage by upstream caspases, such as caspase-6, -8, or -9. The processed form of caspase-3 consists of large (17 kDa) and small (12 kDa) subunits which associate to form the active tetrameric enzyme tetramer (a pair of heterodimers). The active caspase-3 proteolytically cleaves and activates other caspases, as well as relevant targets in the cells (e.g., PARP, SREBPs, and DFF). Activation of procaspase-3 stands at a point of convergence for the two major types of apoptosis signaling pathways-those linked to cell surface death receptors and those linked to mitochondrial release of cytochrome c. |
| Product Type | Antibodies Primary |
| Research area | Cell Death |
| Shipping Information | 4°C |
| Size | 100 µg |
| Source / Host | mouse |
| Species Reactivity | human |
| Stability | 1 year |
| Storage | -20°C |
| Substrate / Buffer | 100 μg IgG in 100 μl volume of PBS containing 50% glycerol, pH 7.2. Contains nopreservatives. |
| Technical Specifications | Caspase-3 (also known as CPP32, Yama, apopain) is a key member of the caspase family of cysteine proteases. Caspase-3 exists in cells as an inactive 32 kDa proenzyme. During apoptosis procaspase-3 is processed at aspartate residues by self-proteolysis and/or cleavage by upstream caspases, such as caspase-6, -8, or -9. The processed form of caspase-3 consists of large (17 kDa) and small (12 kDa) subunits which associate to form the active tetrameric enzyme tetramer (a pair of heterodimers). The active caspase-3 proteolytically cleaves and activates other caspases, as well as relevant targets in the cells (e.g., PARP, SREBPs, and DFF). Activation of procaspase-3 stands at a point of convergence for the two major types of apoptosis signaling pathways-those linked to cell surface death receptors and those linked to mitochondrial release of cytochrome c. |
| Product Page Updated | 2023-12-29T16:20:16.638Z |
