Secondary Reagents

10 nm Colloidal Gold Conjugated Protein A

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10 nm Colloidal Gold Conjugated Protein A

Article No

OABB00906-0.1

Application

EM, IHC, ICC

Size

0.1 mg

Shipping Information

Room Temperature

Documentation

Suggested protocols

Specifications

Additional Information This product may be available in more sizes. Please inquire for size options.
Application EM, IHC, ICC
Article No OABB00906-0.1
Country Availability SE, FI, DK, NO, IS, EE, LV, LT, FO, GL, RU
Description 10 nm Colloidal Gold Conjugated Protein A
Supplier Aviva Systems Biology
Keywords Others;
Notes Protein A is the component of the cytoderm of staphylococcus aureus. It was regarded as a kind of broad spectrum secondary antibody, because it can bind Fc fragments of a majority of immunoglobulins. It can react with guinea pig, rabbit globulin and human IgG1, 2, 4 strongly and react with mouse, bovine globulin and human IgA, E, M weakly.
Product Type Secondary Reagents
Protocol Recommended Dilutions:<br/>EM 1:20-50<br/>IHC (Paraffin-fixed sections) 1:50-100<br/>IHC (frozen-fixed sections)1:50-100<br/>ICC 1:50-100 <br/>Optimal working dilutions must be determined by end user
Shipping Information Room Temperature
Size 0.1 mg
Storage Upon receipt, store product at 4°C. 10 nm Colloidal Gold Conjugated Protein A is stable for one year.
Substrate / Buffer Concentrated, Liquid. 0.1 mg of colloidal gold conjugated protein A, the diameter of colloidal gold is 10+/- 2 nm; 0.01M PBS; 0.01% Thimerosal, 50% glycerol.
Technical Specifications Protein A is a surface protein originally found in the cell wall of the bacteria Staphylococcus aureus. It is encoded by the spa gene and its regulation is controlled by DNA topology, cellular osmolarity, and a two-component system called ArlS-ArlR. It has found use in biochemical research because of its ability to bind immunoglobulins. It is composed of five homologous Ig-binding domains that fold into a three-helix bundle. Each domain is able to bind proteins from many mammalian species, most notably IgGs. It binds the heavy chain within the Fc region of most immunoglobulins and also within the Fab region in the case of the human VH3 family. Through these interactions in serum, where IgG molecules are bound in the wrong orientation (in relation to normal antibody function), the bacteria disrupts opsonization and phagocytosis. Recombinant Protein A is produced in E.coli and essentially functions the same as native Protein A. Protein A may be conjugated with various reporter molecules, including fluorescent dyes (FITC), enzyme markers (peroxidase, b-galactosidase, and alkaline phosphatase), biotin, and colloidal gold without affecting the antibody binding site on the molecule. These conjugates are used to detect immunoglobulins in various immunochemical assays including Western blotting, immunohistochemistry, and ELISA applications.br> Protein A is conjugated to 10 nm colloidal gold. This gold conjugated protein A generates electron dense particles that are visible using electron microscopy.
Product Page Updated 2021-03-12T13:01:35.484Z

Shipping info

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