Proteins & Peptides

Recombinant SARS-CoV-2 Spike glycoprotein

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Recombinant SARS-CoV-2 Spike Glycoprotein

Article No

OPCA335987-1

Application

Electrophoresis

Size

1 mg

Source / Host

yeast

Specifications

MW 38 kDa
NCBI Number 43740568
Application Electrophoresis
Article No OPCA335987-1
Country Availability SE, FI, DK, NO, IS, EE, LV, LT, FO, GL, RU
Description Recombinant SARS-CoV-2 Spike Glycoprotein
Supplier Aviva Systems Biology
Notes Spike glycoprotein comprises two functional subunits responsible for binding to the host cell receptor (S1 subunit) and fusion of the viral and cellular membranes (S2 subunit). For many coronavirus (CoVs), S is cleaved at the boundary between the S1 and S2 subunits, which remain non-covalently bound in the prefusion conformation. The distal S1 subunit comprises the receptor-binding domain(s) and contributes to stabilization of the prefusion state of the membrane-anchored S2 subunit that contains the fusion machinery. S is further cleaved by host proteases at the so-called S2' site located immediately upstream of the fusion peptide in all CoVs. This cleavage has been proposed to activate the protein for membrane fusion via extensive irreversible conformational changes. However, different CoVs use distinct domains within the S1 subunit to recognize a variety of attachment and entry receptors, depending on the viral species. Endemic human coronaviruses OC43 and HKU1 attach via their S domain A to 5-N-acetyl-9-O-acetyl-sialosides found on glycoproteins and glycolipids at the host cell surface to enable entry into susceptible cells. MERS-CoV S uses domain A to recognize non-acetylated sialoside attachment receptors, which likely promote subsequent binding of domain B to the entry receptor, dipeptidyl-peptidase 4. SARS-CoV and several SARS-related coronaviruses (SARSr-CoV) interact directly with angiotensin-converting enzyme 2 (ACE2) via SB to enter target cells.
Alias Names SARS-CoV-2 S1-RBD protein, NCP-CoV RBD Protein, novel coronavirus RBD Protein, 2019-nCoV RBD Protein, S glycoprotein Subunit1 RBD Protein, COVID-19
Product Type Proteins & Peptides
Purification Affinity purified using IMAC
Purity Greater than 90% as determined by SDS-PAGE.
Research Area Virology, Microbiology
Sequence Partial Length (319-541aa): RVQPTESIVRFPNITNLCPFGEVFNATRFASVYAWNRKRISNCVADYSVLYNSASFSTFKCYGVSPTKLNDLCFTNVYADSFVIRGDEVRQIAPGQTGKIADYNYKLPDDFTGCVIAWNSNNLDSKVGGNYNYLYRLFRKSNLKPFERDISTEIYQAGSTPCNGVEGFNCYFPLQSYGFQPTNGVGYQPYRVVVLSFELLHAPATVCGPKKSTNLVKNKCVNF
Size 1 mg
Source / Host yeast
Storage We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL. We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20C/-80C. Store working aliquots at 4C for up to one week. Our default final concentration of glycerol is 50%. Repeated freezing and thawing is not recommended.
Substrate / Buffer Lyophilized from Tris/PBS-based buffer, 6% Trehalose, pH 8.0
Product Page Updated 2021-03-08T08:29:25.844Z

Shipping info

The delivery time for this item is approximately 8-16 business days. If other deviations occur, this will be noted on the order confirmation or communicated via email. Please note, we do reserve the right to select the best packaging and shipping method in order to insure the stability of the product and allow efficient order tracking.