14-3-3 sigma

Article No PAT-80410-2
Product Type Proteins & Peptides
Research Area Cell Death
Application Electrophoresis
Size 0.5mg

Specifications

MW 27.7 kDa (248aa), confirmed by MALDI-TOF.
NCBI Number NP_006133
Country Availability all
BioSite Brand BioSite
Concentration 1 mg/ml (determined by Bradford assay)
Description 14-3-3 sigma, 1-248aa, Human, E.coli
Supplier BioSite
Notes 14-3-3 sigma ,also known as Stratifin (SFN), belong to the 14-3-3 family. The 14-3-3 family of proteins plays a key regulatory role in signal transduction, checkpoint control, apoptotic and nutrient-sensing pathways. 14-3-3 proteins are highly conserved and ubiquitously expressed. There are at least seven isoforms, beta, gamma, epsilon, sigma, zeta, tau and eta that have been identified in mammals. 14-3-3 sigma was identified as an epithelial cell marker and appeared to function as a tumor suppressor whose expression can be down regulated via methylation. Loss of 14-3-3 sigma expression results in a defective G2/M phase checkpoint and appears to contribute to both epithelial and non-epithelial tumorigenesis. Recombinant human 14-3-3 sigma was expressed in E.coli and purified by using conventional chromatography techniques.
Alias Names stratifin, Epithelial cell marker protein 1, HME1, YWHAS, SFN, 14-3-3 sigma
Purity > 95% by SDS - PAGE
Sequence MERASLIQKA KLAEQAERYE DMAAFMKGAV EKGEELSCEE RNLLSVAYKN VVGGQRAAWR VLSSIEQKSN EEGSEEKGPE VREYREKVET ELQGVCDTVL GLLDSHLIKE AGDAESRVFY LKMKGDYYRY LAEVATGDDK KRIIDSARSA YQEAMDISKK EMPPTNPIRL GLALNFSVFH YEIANSPEEA ISLAKTTFDE AMADLHTLSE DSYKDSTLIM QLLRDNLTLW TADNAGEEGG EAPQEPQS
Source / Host bacteria
Species Reactivity human
Storage Can be stored at +4C short term (1-2 weeks). For long term storage, aliquot and store at -20C or -70C. Avoid repeated freezing and thawing cycles.
Substrate / Buffer Liquid. In 20mM Tris-HCl buffer (pH8.0) containing 50mM NaCl, 10% glycerol
Product Page Updated 2018-10-30T15:53:36.445Z

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Source/Host:

bacteria

Species reactivity:

human human