Article No
PM020
Specifications
| Application | ICC, IP, WB |
| Article No | PM020 |
| Country Availability | SE, FI, DK, NO, IS, EE, LV, LT, FO, GL |
| Clone Type | polyclonal |
| Conjugation | Unconjugated |
| Description | Anti-DDDDK-tag pAb |
| Supplier | MBL |
| Immunogen | Synthetic peptide binding KLH, DYKDDDDK |
| Isotype | IgG |
| Notes | Epitope tagging is a powerful and versatile strategy for detecting and purifying proteins expressed by cloned genes. Short sequences encoding the epitope tag are cloned in-frame with target DNA to produce fusion proteins containing the epitope tag peptide. Due to their small size, epitope tags do not affect the tagged protein’s biochemical properties. Anti-epitope tag antibodies can serve as universal purification or detection reagents for any tag-containing protein. The DDDDK epitope tag peptide sequence (XXXDDDDK) was first derived from the 11-amino-acid leader peptide of the gene-10 product from bacteriophage T7. The DDDDK peptide has been widely used as a multi-purpose tag, and anti-DDDDK antibodies are optimally suited for identifying, detecting, purifying, and monitoring the expression levels of recombinant DDDDK fusion proteins. |
| Product Type | Antibodies Primary |
| Shipping Information | 4°C |
| Size | 100 µg |
| Source / Host | rabbit |
| Stability | 1 year |
| Storage | -20°C |
| Substrate / Buffer | 100 μl volume of PBS containing 50% glycerol, pH 7.2. Contains no preservative. |
| Technical Specifications | Epitope tagging is a powerful and versatile strategy for detecting and purifying proteins expressed by cloned genes. Short sequences encoding the epitope tag are cloned in-frame with target DNA to produce fusion proteins containing the epitope tag peptide. Due to their small size, epitope tags do not affect the tagged protein’s biochemical properties. Anti-epitope tag antibodies can serve as universal purification or detection reagents for any tag-containing protein. The DDDDK epitope tag peptide sequence (XXXDDDDK) was first derived from the 11-amino-acid leader peptide of the gene-10 product from bacteriophage T7. The DDDDK peptide has been widely used as a multi-purpose tag, and anti-DDDDK antibodies are optimally suited for identifying, detecting, purifying, and monitoring the expression levels of recombinant DDDDK fusion proteins. |
| Product Page Updated | 2023-12-29T16:20:16.638Z |
