Article No
RN004P
Anti-ELAVL1 (HuR) (Human) pAb
Anti-ELAVL1 (HuR) (Human) pAb
Antibodies Primary
Specifications
| Application | IP, RIA, WB, ICC |
| Article No | RN004P |
| Country Availability | SE, FI, DK, NO, IS, EE, LV, LT, FO, GL |
| Clone Type | polyclonal |
| Concentration | 1 mg/mL |
| Conjugation | Unconjugated |
| Description | Anti-ELAVL1 (HuR) (Human) pAb |
| Supplier | MBL |
| Additional Information | Posttranscriptional regulation of gene expression is a ribonucleoprotein-driven process, which involves RNA binding proteins (RBPs) and non-coding RNAs that affect splicing, nuclear export, subcellular localization, mRNA decay and translation. The RNP Immunoprecipitation-Chip (RIP-Chip), RIP-Seq and RIP-RTPCR allow the identification of multiple RNA targets of RBPs globally and within the context of a cell extract. Antibodies specific to the RNA binding protein of interest are used to co-immunoprecipitate the RNA binding protein and the associated subset of mRNAs. The mRNA content is interrogated using standard microarray or sequencing technology. RIP-Certified Antibody is validated for use in RNP Immunoprecipitation (RIP) in conjunction with the RIP-Assay kit distributed from MBLI. Its ability to immunoprecipitate mRNAs and RBPs complex was confirmed by quantitative and qualitative analysis on NanoDrop, Bioanalyzer and RT-PCR or microarray. |
| Immunogen | Peptide bond synthesis MSNGYEDHMAEDCRGDIGR KLH (1-19 aa) |
| Notes | ELAVL1/HuR, like other ELAVL proteins, has a short N-terminal region followed by 2 RNA-binding motifs, a basic linker domain, and a third RNA-binding motif. The basic hinge region bears a novel nuclear shuttling sequence which is implicated in the nucleocytoplasmic HuR shuttling. Unlike its neuron-specific ELAV relatives (HuB, HuC and HuD), HuR itself is ubiquitously expressed. Structure-function analyses of HuR have shown that it binds to AU-rich mRNA sequences in the nucleus and may be involved in their nuclear export. |
| Product Type | Antibodies Primary |
| Research area | Epigenetics |
| Shipping Information | 4°C |
| Size | 200 µl |
| Source / Host | rabbit |
| Species Reactivity | human, mouse, rat, hamster |
| Stability | 1 year |
| Storage | -20°C |
| Substrate / Buffer | 200 μl volume of PBS containing 50% glycerol, pH 7.2. No preservative is contained. |
| Technical Specifications | ELAVL1/HuR, like other ELAVL proteins, has a short N-terminal region followed by 2 RNA-binding motifs, a basic linker domain, and a third RNA-binding motif. The basic hinge region bears a novel nuclear shuttling sequence which is implicated in the nucleocytoplasmic HuR shuttling. Unlike its neuron-specific ELAV relatives (HuB, HuC and HuD), HuR itself is ubiquitously expressed. Structure-function analyses of HuR have shown that it binds to AU-rich mRNA sequences in the nucleus and may be involved in their nuclear export. |
| Product Page Updated | 2023-12-29T16:20:16.638Z |
