Article No
RN024P
Anti-PCBP1 (Human) pAb
Anti-PCBP1 (Human) pAb
Antibodies Primary
Specifications
| Application | IP, RIA, WB |
| Article No | RN024P |
| Country Availability | SE, FI, DK, NO, IS, EE, LV, LT, FO, GL |
| Clone Type | polyclonal |
| Concentration | 1 mg/mL |
| Conjugation | Unconjugated |
| Description | Anti-PCBP1 (Human) pAb |
| Supplier | MBL |
| Additional Information | Posttranscriptional regulation of gene expression is a ribonucleoprotein-driven process, which involves RNA binding proteins (RBPs) and non-coding RNAs that affect splicing, nuclear export, subcellular localization, mRNA decay and translation. The RNP Immunoprecipitation-Chip (RIP-Chip), RIP-Seq and RIP-RTPCR allow the identification of multiple RNA targets of RBPs globally and within the context of a cell extract. Antibodies specific to the RNA binding protein of interest are used to co-immunoprecipitate the RNA binding protein and the associated subset of mRNAs. The mRNA content is interrogated using standard microarray or sequencing technology. RIP-Certified Antibody is validated for use in RNP Immunoprecipitation (RIP) in conjunction with the RIP-Assay kit distributed from MBLI. Its ability to immunoprecipitate mRNAs and RBPs complex was confirmed by quantitative and qualitative analysis on NanoDrop, Bioanalyzer and RT-PCR or microarray. |
| Immunogen | Internal region synthetic peptide binding KLH, human PCBP1 |
| Isotype | Ig |
| Notes | Poly(rC)-binding proteins (PCBPs) are cellular RNA-binding proteins; these proteins are defined by their triple KH structure and poly(C)-binding specificity. The 3 KH domains are not only involved in nucleic acid binding but also in mediating protein-protein interactions within the RNP complexes. PCBPs bind specifically to polypyrimidine tracts within the 3′ UTR of target mRNAs through the KH domains. PCBPs are expressed as 4 isoforms in cells. PCBP1 and PCBP2 are widely expressed in both the nucleus and the cytoplasm and can shuttle from the nucleus to the cytoplasm, while PCBP3 and PCBP4 are expressed to a less extent. PCBP1 and PCBP2 have been reported to stabilize the target mRNAs such as those of α-globin, α1(I) collagen, and tyrosine hydroxylase by binding to C-rich 3′ UTR motifs. The 15-lipoxygenase mRNA is translationally silent until reticulocytes in the peripheral blood undergo the final steps of maturation. This translational silencing has been shown to be mediated by the binding of PCBP1 and hnRNPK to the 3′ UTR of 15-lipoxygenase mRNA. |
| Product Type | Antibodies Primary |
| Research area | Epigenetics |
| Shipping Information | 4°C |
| Size | 200 µl |
| Source / Host | rabbit |
| Species Reactivity | human, mouse, rat |
| Stability | 1 year |
| Storage | -20°C |
| Substrate / Buffer | 200 μl volume of PBS containing 50% glycerol, pH 7.2. No preservative is contained. |
| Technical Specifications | Poly(rC)-binding proteins (PCBPs) are cellular RNA-binding proteins; these proteins are defined by their triple KH structure and poly(C)-binding specificity. The 3 KH domains are not only involved in nucleic acid binding but also in mediating protein-protein interactions within the RNP complexes. PCBPs bind specifically to polypyrimidine tracts within the 3′ UTR of target mRNAs through the KH domains. PCBPs are expressed as 4 isoforms in cells. PCBP1 and PCBP2 are widely expressed in both the nucleus and the cytoplasm and can shuttle from the nucleus to the cytoplasm, while PCBP3 and PCBP4 are expressed to a less extent. PCBP1 and PCBP2 have been reported to stabilize the target mRNAs such as those of α-globin, α1(I) collagen, and tyrosine hydroxylase by binding to C-rich 3′ UTR motifs. The 15-lipoxygenase mRNA is translationally silent until reticulocytes in the peripheral blood undergo the final steps of maturation. This translational silencing has been shown to be mediated by the binding of PCBP1 and hnRNPK to the 3′ UTR of 15-lipoxygenase mRNA. |
| Product Page Updated | 2023-12-29T16:20:16.638Z |
