Article No
SBB-KP0037-KI01
20S Immunoproteasome Assay Kit
20S Immunoproteasome Assay Kit
Plastics & Consumables
Specifications
| Article No | SBB-KP0037-KI01 |
| Country Availability | SE, FI, DK, NO, IS, EE, LV, LT |
| Components | 10x 20S Immunoproteasome | 50x LLVY-AMC, Chemotrypsin-like activity | 50x PAL-AMC, beta1i/PSMB9 specific substrate | 50x ANW-AMC, beta5i/PSMB8 specific substrate | 50x Inhibitor (ONX-0914, 2mM) in 100% DMSO | 10x Reaction Buffer | 50x SDS(1.75%) in H20 | 100x free AMC Standard (40µM) |
| Description | 20S Immunoproteasome Assay Kit |
| Detection Method | Fluorometric |
| Supplier | Adipogen Life Sciences |
| Format | Powder |
| Notes | South Bay Bio's kit is designed to test for specific activity of 20S immunoproteasome. The kit provides purified 20S immunoproteasome and is designed to test for Chymotrypsin-like activity (Suc-LLVY-AMC) and Caspase-like activity of the immunoproteasome subunits beta1i/ PSMB9 (PAL-AMC) and beta5i/PSMB8 (ANW-AMC). Additionally, the compound, ONX-0914, which can be used to inhibit specifically the subunit beta5i/LMP7 20S immunoproteasome is included. All peptide substrates are conjugated to AMC, which upon proteasome catalyzed hydrolyses display fluorescence at Excitation=345nm, Emission= 445nm; allowing for a real-time read out of 20S immunoproteasome specific activity. |
| Product Type | Plastics & Consumables |
| Shipping Information | DRY ICE |
| Size | 1 Kit |
| Species Reactivity | human |
| Storage | -20°C, -80°C |
| Technical Specifications | South Bay Bio's kit is designed to test for specific activity of 20S immunoproteasome. The kit provides purified 20S immunoproteasome and is designed to test for Chymotrypsin-like activity (Suc-LLVY-AMC) and Caspase-like activity of the immunoproteasome subunits beta1i/ PSMB9 (PAL-AMC) and beta5i/PSMB8 (ANW-AMC). Additionally, the compound, ONX-0914, which can be used to inhibit specifically the subunit beta5i/LMP7 20S immunoproteasome is included. All peptide substrates are conjugated to AMC, which upon proteasome catalyzed hydrolyses display fluorescence at Excitation=345nm, Emission= 445nm; allowing for a real-time read out of 20S immunoproteasome specific activity. |
| Product Page Updated | 2024-02-01T08:25:01.492Z |
