Whole cell lysates of the shown cell lines were separated by 7.5% SDS-PAGE and transferred to nitrocellulose membrane. After blocking in 3% NFDM in PBS-T, the membrane was incubated with purified CRISPR/Cas9 (SaCas9) Monoclonal Antibody [6H4] (1:10000 diluted) at 4°C o/n and Cat. #A12003 for 1 hour at room temp. Protein expressions were visualized using ECL exposed to X-ray film.
EpiGentek is pleased to now offer the CRISPR/Cas9 (SaCas9) Monoclonal Antibody [6H4] which specifically recognizes Cas9 and dCas9 (nuclease deficient Cas9).
Staphylococcus aureus Cas9 (SaCas9)-mediated genome editing has been reported in both human cells and Arabidopsis. Because SaCas9 (1053 a.a.) is smaller than the more commonly used Streptococcus pyogenes Cas9 (SpCas9) (1368 a.a.), SaCas9 could have substantial advantages for delivering and expressing Cas9 protein, especially using virus vectors.
This antibody is now being offered at an extra 15% off through December 31, 2017. This promotion cannot be used in conjunction with any other promotional offer.