Article No
36-2518-100
Application | FC, IF, IHC |
Article No | 36-2518-100 |
Country Availability | SE, FI, DK, NO, IS, EE, LV, LT |
Clone | 101-1D2 |
Clone Type | monoclonal |
Conjugation | Unconjugated |
Description | Anti-CD50 / ICAM3 Monoclonal Antibody(Clone: 101-1D2) |
Supplier | Abeomics |
Entrez Gene ID | 3385 |
Gene Symbol | ICAM3 |
Immunogen | Stimulated human leukocytes |
Isotype | IgG2a k |
Notes | Recognizes an N-glycosylated glycoprotein of 120kDa with intra-chain disulfide bonds, identified as CD50 or ICAM-3 (WS: IV V). Its epitope localizes in the D2 extracellular domain and is resistant to neuraminidase and proteases. CD50 is the major ligand for LFA-1 (CD11a/CD18) and may have signalling role to increase adhesion. It is expressed on thymocytes and T lymphocytes and is resistant to treatment with phosphatidylinositol (PI) phospholipase C. This MAb inhibits primary mixed lymphocyte culture (MLC) but not secondary MLC, cytotoxicity or proliferation induced by mitogens. It blocks binding of NK1-L16 stimulated T cells to L cells expressing CD50. This MAb is excellent for staining of formalin/paraffin tissues. |
Alias Names | ICAMR; Intercellular adhesion molecule 3 (ICAM3) |
Product Type | Antibodies Primary |
Protocol | Flow Cytometry (1-2ug/million cells); Immunofluorescence (1-2ug/ml); Immunohistochemistry (Formalin-fixed) (1-2ug/ml for 30 minutes at RT)(Staining of formalin-fixed tissues requires heating tissue sections in 10mM Tris with 1mM EDTA, pH 9.0, for 45 min at 95°C followed by cooling at RT for 20 minutes) |
Shipping Information | Blue Ice |
Size | 100 µg |
Source / Host | mouse |
Species Reactivity | human |
Storage | 4°C, -80°C |
Substrate / Buffer | 200 µg/ml of Ab Purified from Bioreactor Concentrate by Protein A/G. Prepared in 10mM PBS with 0.05% BSA & 0.05% azide. Also available WITHOUT BSA & azide at 1.0mg/ml. |
UniProt Number | P32942 |
Product Page Updated | 2024-02-06T07:35:53.072Z |